strain s aureus atcc 35556  (ATCC)

Journal: BMC Microbiology

Article Title: Repurposing Etalocib suppresses multidrug-resistant Staphylococcus aureus by disrupting the bacterial membrane

doi: 10.1186/s12866-025-04163-5

Figure Lengend Snippet: The antibacterial activity of Etalocib in vitro. A Chemical structure of Etalocib. B Effects of different concentrations of Etalocib (1/16 ×, 1/8 ×, 1/4 ×, 1/2 × and 1 × MIC) on the bacterial growth of MRSA and MSSA planktonic cells. C Time-kill curve of Etalocib with various concentrations (1 ×, 2 × and 4 × MIC) against the exponential phase of MSSA and MRSA. The MIC values of Etalocib against MRSA YUSA139, MSSA CHS101, and MSSA SA113 were 6.25 μM. The MIC values of Etalocib against MRSA YUSA145 was 12.5 μM. The MIC value of vancomycin against MRSA and MSSA were 1 μg/mL. For ( B - D ), n = 3 and data were mean ± SD

Article Snippet: The reference strain S. aureus SA113 (ATCC35556) purchased from American Type Culture Collection (ATCC), and was used as the quality control of antimicrobial susceptibility strain.

Techniques: Activity Assay, In Vitro

Journal: BMC Microbiology

Article Title: Repurposing Etalocib suppresses multidrug-resistant Staphylococcus aureus by disrupting the bacterial membrane

doi: 10.1186/s12866-025-04163-5

Figure Lengend Snippet: Inhibitory and bactericidal effects of Etalocib on the biofilm. A - C Effect of sub-inhibitory concentrations (1/8 ×, 1/4 ×, and 1/2 × MIC) of Etalocib on the biofilm formation of MSSA ( A ), MRSA ( B ) and E . faecalis ( B ) by crystal violet staining. The MIC values of Etalocib against EF16C168 were 3.13 μM. The MIC values of Etalocib against SA113, CHS101, CHS727, YUSA139, EF16C166, EF16C350 and EF16C353 were 6.25 μM. The MIC values of Etalocib against YUSA143, YUSA145 were 12.5 μM. The MIC values of Etalocib against YUSA21, YUSA50 were 25 μM. The MIC values of LZD against CHS727, YUSA145 and YUSA21 were 1 μg/mL. The MIC values of LZD against YUSA143, YUSA139, CHS101, SA113, YUSA50, EF16C166, EF16C168, EF16C350 and EF16C353 were 2 μg/mL. LZD, Linezolid. D - I Impact of different concentrations of Etalocib on S. aureus YUSA145 embedded in the mature biofilm was investigated by CLSM. Living cells and dead cells were labeled with SYTO 9 (green) and PI (red) dyes, respectively. Van: Vancomycin, as a positive control. Scale bar = 20 μm. For ( A ), n = 3 and data were presented by mean ± SD, two-way ANOVA with Dunnett’s multiple comparisons test. * P < 0.05, ** P < 0.01, *** P < 0.001, *** P < 0.0001

Article Snippet: The reference strain S. aureus SA113 (ATCC35556) purchased from American Type Culture Collection (ATCC), and was used as the quality control of antimicrobial susceptibility strain.

Techniques: Staining, Labeling, Positive Control

Journal: Scientific Reports

Article Title: Assessment of the biofilm formation capacities of Staphylococcus aureus strains Newman and Newman D2C in vitro and in vivo

doi: 10.1038/s41598-025-00521-5

Figure Lengend Snippet: Biofilm formation of S. aureus strains SA113, Newman HOM, and Newman D2C HOM in TSB supplemented with 0.5% glucose. Exponential growth phase cells of strains SA113 (red bars), Newman HOM (orange bars), and Newman D2C HOM (turquoise bars) were inoculated into TSB supplemented with 0.5% glucose (TSB-G) and cultured in Nunclon Delta-treated 96-well microplates for 18 h at 37 °C under static or dynamic (120 rpm) conditions as indicated. ( a–d ) Vegetation were washed twice prior to the safranin staining ( a , b ) or washed as indicated ( c , d ). ( a , c ) Representative images of safranin-stained vegetation. ( b , d ) A 530 readings of safranin contents in the wells after solubilization with 30% acetic acid. ( e ) Impact of the washing steps on the A 530 readings of safranin contents in the wells. Values are given in relation to the safranin signals seen in wells that were not washed, which were set to 100%. ( f ) PIA contents of the vegetation formed by strains SA113, Newman HOM, and Newman D2C HOM in TSB-G in 96-well microplates cultured for 18 h at 37 °C under static conditions. PIA contents of the biofilms were determined by staining the vegetation with XFD488-labeled WGA (2.5 µg/ml) and determining the fluorescence signals of the incorporated dye at 480/530 nm (Excitation/Emission). Differences in PIA contents are given as relative light units (RFUs). Results represent the averages of five to nine independent experiments done in duplicate. Error bars indicate the standard deviation of the mean. Round symbols indicate the mean A 530 /%/RFU values of individual experiments. * p < 0.05; ** p < 0.01 (Kruskal–Wallis test and Dunn’s multiple comparison test).

Article Snippet: A major negative impact of agr on biofilm formation of S. aureus is also suggested by the fact that many S. aureus isolates forming a strong PIA-dependent biofilm under in vitro conditions display low to no agr expression , , and that the strong PIA-dependent biofilm-producing strain SA113 (ATCC 35556) is a natural agr mutant.

Techniques: Cell Culture, Staining, Labeling, Fluorescence, Standard Deviation, Comparison

Journal: Scientific Reports

Article Title: Assessment of the biofilm formation capacities of Staphylococcus aureus strains Newman and Newman D2C in vitro and in vivo

doi: 10.1038/s41598-025-00521-5

Figure Lengend Snippet: Three-dimensional organization and PIA distribution of the vegetation formed by strains SA113, Newman HOM, and Newman D2C HOM in PS-based microplates. Exponential growth phase cells of S. aureus strains were inoculated into TSB supplemented with 0.5% glucose and cultured in tissue culture-treated, PS-based 6-well microplates for 18 h at 37 °C under static conditions. The vegetation formed were stained with XFD488-WGA and Nile Red (NR), and fluorescence was monitored with CLSM. ( a , b ) Three-dimensional image reconstructions of z series were recorded at 525 nm (XFD488) and 595 nm (NR), respectively. Reconstructed top view ( a ) and side view ( b ) images of the vegetation formed by the test strains. CLSM reconstructions are representative of three separate experiments. Each side of a grid square in the image reconstructions represents 100 μm. Scale bar, 200 μm (scale bar applies to all images in the respective panel).

Article Snippet: A major negative impact of agr on biofilm formation of S. aureus is also suggested by the fact that many S. aureus isolates forming a strong PIA-dependent biofilm under in vitro conditions display low to no agr expression , , and that the strong PIA-dependent biofilm-producing strain SA113 (ATCC 35556) is a natural agr mutant.

Techniques: Cell Culture, Staining, Fluorescence

Journal: Scientific Reports

Article Title: Assessment of the biofilm formation capacities of Staphylococcus aureus strains Newman and Newman D2C in vitro and in vivo

doi: 10.1038/s41598-025-00521-5

Figure Lengend Snippet: Biofilm formation of S. aureus strains SA113, Newman HOM, and Newman D2C HOM in TSB-HBS or RPMI-1640 under static conditions. ( a , b ) Exponential growth phase cells of strains SA113 (red bars), Newman HOM (orange bars), and Newman D2C HOM (turquoise bars) were inoculated into TSB supplemented with 5% human blood serum (TSB-HBS; a ) or Roswell Park Memorial Institute 1640 medium (RPMI-1640; b ) and cultured in Nunclon Delta-treated 96-well microplates for 18 h at 37 °C under static conditions. Washing steps prior to the safranin staining were performed as indicated. ( a , c ) Representative images of safranin-stained vegetation. ( b , d ) A 530 readings of safranin contents in the wells after solubilization with 30% acetic acid. Results represent the averages of six to nine independent experiments done in duplicate. Error bars indicate the standard deviation of the mean. Round symbols indicate the mean A 530 values of individual experiments. * p < 0.05; ** p < 0.01 (Kruskal–Wallis test and Dunn’s multiple comparison test).

Article Snippet: A major negative impact of agr on biofilm formation of S. aureus is also suggested by the fact that many S. aureus isolates forming a strong PIA-dependent biofilm under in vitro conditions display low to no agr expression , , and that the strong PIA-dependent biofilm-producing strain SA113 (ATCC 35556) is a natural agr mutant.

Techniques: Cell Culture, Staining, Standard Deviation, Comparison

Journal: Scientific Reports

Article Title: Assessment of the biofilm formation capacities of Staphylococcus aureus strains Newman and Newman D2C in vitro and in vivo

doi: 10.1038/s41598-025-00521-5

Figure Lengend Snippet: Biofilm formation of S. aureus strains SA113, Newman HOM, and Newman D2C HOM on PVC tubing under dynamic conditions. ( a ) Cell suspensions of strains SA113 (red bars), Newman HOM (orange bars), and Newman D2C HOM (turquoise bars) were used to inoculate the lumen of 1 cm long PU-based PVC tubing fragments, and the infected tubing fragments were cultured in TSB or RPMI-1640 for 5 days at 37 °C under non-nutrient limiting and dynamic conditions (rotation at 20 rpm). ( b ) Biofilm formation of S. aureus strains SA113, Newman HOM, and Newman D2C HOM on the lumen of PVC tubing in TSB under constant flow (1 ml/min). CFU rates of detached biofilms are shown. Error bars indicate the standard deviation of the mean. Round symbols indicate the CFU values of individual experiments. ns not significant; * p < 0.05 (Kruskal–Wallis test and Dunn’s multiple comparison test).

Article Snippet: A major negative impact of agr on biofilm formation of S. aureus is also suggested by the fact that many S. aureus isolates forming a strong PIA-dependent biofilm under in vitro conditions display low to no agr expression , , and that the strong PIA-dependent biofilm-producing strain SA113 (ATCC 35556) is a natural agr mutant.

Techniques: Infection, Cell Culture, Standard Deviation, Comparison